Of increasing concern is the level of carbapenem resistance amongst Gram-negative bacteria caused by carbapenemases.
Of increasing concern is the level of carbapenem resistance amongst Gram-negative bacteria caused by carbapenemases.
Although there are commercial solutions for the detection of carbapenemases in Enterobacteriaceae, options for Pseudomonas and Acinetobacter spp. remain limited.
The MAST ICT method utilises a cell permeabilising agent to release carbapenemase enzyme from a carbapenemase-producing organism (CPO) to hydrolyse an indicator antibiotic in the test system.
It is a reliable screening tool for OXA, KPC, NDM, IMP and VIM enzymes and suitable for use with Acinetobacter, Pseudomonas spp and Enterobacteriaceae.
